Technical Data
M4470-12
Monocarboxylate Transporter 2 (MCT2, MOT2)
Description:
Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
MCT2/MOT2 (mouse 484 aa, rat 489 aa, human 478 aa, chromosome 12q13) is less widely distributed than MCT. It is associated with tissues that demonstrate a high uptake affinity for lactate and pyruvate such as the kidney and liver (for gluconeogenesis) and neurons (for oxidation). It may function in transporting lactate from intestine and erythrocytes. MCT1 is most closely related to MCT1 (~55% identity, whereas homolog with other MCT1-MCT8 isoforms is less (~35-53%). Both N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-5ug/ml using ECL.
ELISA: 1:10,000-1:100,000 Using 50-100ng M4470-12A control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to 15aa near the C-terminal, cytoplasmic domain of human MCT2, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes human Monocarboxylate Transporter 2 (MCT2). The immunogen shows no significant sequence homology with other MCT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Wang, Q. et al., (2007) Pharm Res. 24:1067-1078. 1) Jackson VN et al (1997) Biochem. J. 324:447453; Dao L et al (1998) J. Biol. Chem. 273:2895928965; Koehler-Stec EM et al (1998) Am. J. Physiol. 275:E516-E524; Price NT et al (1998) Biochem. J. 329:321328 (review); Halestrap AP and Price NT (1999) Biochem J. 343:281299 (review)