Technical Data
M4590-04A
MOP3 (Members of PAS Superfamily, BMAL1, Brain And Muscle ARNT-like 1, JAP3, PAS3, CYCLE)
Description:
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257 aa) and Per3 (1113 aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Mouse Clock (855 aa) is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery. Basic-helix-loop-helix-PAS orphan protein, MOP3 (Members Of PAS Superfamily; also known as BMAL1/JAP3/PAS3; 626 aa) is a general dimerization partner for several PAS superfamily of transcription regulators. MOP3 interacts with MOP4 (also known as NPAS2; 626 aa), Clock, HIF1a, and HIF2a. MOP4 is a brain specific homolog of Clock.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceDrosophilaRabbit
Concentration:
As reported
Immunogen:
A 22-aa peptide sequence within the N-terminus of Drosophila BMAL1 (413 aa, refs 1) was synthesized, coupled to KLH, and generated in rabbits
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Specificity:
Drosophila sequence has no significant sequence homology with mammalian Clock or Per proteins. Significant sequence homology of the sequence is seen with mammalian USFs (Upstream Stimulatory Factors 1 and 2) and human and mouse BMAL1 and ARNTs. Antibody crossreactivity with these proteins has not been not tested. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1.) Darlington TK et al (1998) Science 280, 1599-1603; Rutila JE et al (1998) Cell 93, 805-814; Bae K et al (1998) Gene accession # 3219728.