Technical Data
MRP5 (Multidrug Resistance-associated Protein 5, ABC33, ATP-binding Cassette Sub-family C Member 5, ABCC5, DKFZp686C1782, EST277145, Multi-specific Organic Anion Transporter C, MOATC, MOAT-C, pABC11, SMRP)
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear.

Suitable for use in Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry (acetone fixed): 1:20-50
Immunohistochemistry (acetone fixed frozen): 1:20
Western Blot: 1:20-50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceRat
~250ug immunoglobulin/ml
Bacterial fusion protein of MRP5, containing aa82-168 of the protein.
Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.
Recognizes an internal epitope of MRP5, a 190-200kD transmembrane protein that is closely related to the multidrug resistance protein MRP. Does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kool et al. Analysis of expression of cMOAT (MRP2), MRP3, MRP4 and MRP5, homologs of the multidrug resistance associated protein gene (MRP1), in human cancer cell lines Cancer res. 57: 3537-3547, 1997 2. Scheffer et al. Specific detection of multidrug resistance proteins MRP1, MPR2, MRP3, MRP5 and MDR3 p-Glycoprotein with a panel of monoclonal antibodies. Cancer Research, 60, 5269-77, 2000.