Technical Data
Media
m-TEC Agar (Powder)
M4693
Microbiological Grade
Storage RT/4C    Shipping RT
Formula per Liter
Enzymatic Digest of Animal Tissue5g
Yeast Extract3g
Lactose10g
Sodium Chloride7.6g
Potassium Pjosphate4.3g
Sodium Lauryl Sulfate0.2g
Sodium Deoxycholate0.1g
Bromcresol Purple0.08g
Bromphenol Reb m0.08g
Agar15g
Final pH: 7.3 +/- 0.2 at 25C.
m-TEC Agar is used with urea for the isolation and enumeration of thermotolerant E. coli from water using the membrane filtration technique. E. coli is used as an indicator of fecal pollution in water. Researchers have been able to to quantitate E. coli on m-TEC Agar with 24 hours without requiring subculture and identification of isolates. m-TEC is an acronymn for membrane thermotolerant E. coli.


m-TEC Agar 1. Suspend 45.3g of the medium in 1.0L high purity water.

2. Heat with frequent agitation and boil to completely dissolve the medium. 3. Autoclave at 121C for 15 minutes.4. Dispense 4-5 ml amounts into 10 x 50mm petri dishes and allow to solidify.Urea Substrate1. Combine 2g urea and 10mg phenol red in 100ml high purity water.2. Adjust pH to 5.0 +/- 0.3.3. Store at 2-8C. Use within 1 week.

Enzymatic digest of animal tissue provides nitrogen, carbon and minerals in m-TEC Agar. Yeast extract is a source of vitamins and trace elements. Lactose serves as a carbon source. Potassium Phosphate is the buffering agent. Sodium Lauryl Sulfate and Sodium Deoxycholate are selective agents against gram-positive bacteria. Bromcresol Purple and Bromphenol Red are pH indicators. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.:

Apparance: Light grey-green-beige homogeneous, free-flowing powder

Solubility: Dark purple, trace to slightly hazy

pH: 7.30.2 . Expected Cultural Responses. Response on m-TEC Agar at 44.5C after 202 hours incubation. Yellow to yellow-brown colonies (urease-negative) may be presumptively identified as E. coli. Response Reaction w/ Urease Substrate Enterococcus faecalis (ATCC 29212) inhibited - E. coli (ATCC 8738) growth yellow colonies E. coli (ATCC 35150) growth yellow colonies E. Coli (ATCC 35218) growth yellow colonies Pseudomonas aeruginosa inhibited - (ATCC 27853)

Storage and Stability. Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time. :


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.