Technical Data
Mucin 1 (MUC1 Glycoprotein, CD227)
Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and paraffin sections).

Recommended Dilution:
Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature.

Antigen Localization:
Cytoplasm and membrane.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20CBlue IceHumanMouse
As reported
Human milk fat globuline membrane preparation.
Purified IgG
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Reactive with 90% of breast carcinoma, most epithelial ovarian carcinoma and a high portion of the lung, urogenital and gastrointestinal carcinomas. Some reactivity is observed in the apical membrane of normal breast epithelium. Weak reactivity is also present in pancreas, kidney and lung. Reacts with a five amino acid epitope of the MUC-1 core protein which is less effective for glysosilation than most other MUC-1 epitopes. Recommended for Mamma carcinoma. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Xing PX et al. Monoclonal Antibodies reactive with mucin expressed in breast cancer (1989). Immunol Cell Biol 67, 183-195 Mc Guckin MA et al. Occult axillary node metastases in breast cancer: their detection and prognostic significance (1996). British Journal of Cancer 73, 88-85