Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and paraffin sections).
Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature.
Cytoplasm and membrane.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|Human milk fat globuline membrane preparation.|
|Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.|
|Reactive with 90% of breast carcinoma, most epithelial ovarian carcinoma and a high portion of the lung, urogenital and gastrointestinal carcinomas. Some reactivity is observed in the apical membrane of normal breast epithelium. Weak reactivity is also present in pancreas, kidney and lung. Reacts with a five amino acid epitope of the MUC-1 core protein which is less effective for glysosilation than most other MUC-1 epitopes. Recommended for Mamma carcinoma. Species Crossreactivity: Human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Xing PX et al. Monoclonal Antibodies reactive with mucin expressed in breast cancer (1989). Immunol Cell Biol 67, 183-195 Mc Guckin MA et al. Occult axillary node metastases in breast cancer: their detection and prognostic significance (1996). British Journal of Cancer 73, 88-85|