Technical Data
M9520-12A
Muscarinic Acetylcholine Receptor M2, aa251-351 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)
Description:
The muscarinic acetylcholine receptor belongs to the G-protein coupled receptor 1 family and mediates various cellular responses such as adenylate cyclase inhibition, phosphoinositide breakdown and potassium channel modulation through the action of G proteins. The unprocessed precursor has a length of 466aa and a predicted molecular weight of 51.7KD.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: Detection limit of recombinant GST tagged muscarinic acetylcholine receptor is 0.03ng/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20C. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G384Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Partial recombinant protein of human muscarinic acetylcholine receptor (aa 251-351) with a GST tag.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2 Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human Muscarinic Acetylcholine Receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.