Technical Data
E. coli-derived MutS is a protein component of the mismatch repair pathway, selectively binding DNA fragments containing mispaired or unpaired bases. MutS binds mismatches that most resemble base pairs (i.e., G:T mismatches in G:C rich regions), allowing MutS to be used for mutation/SNP detection and isolation applications and mismatch repair structure/function studies.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:10,000; Recommended dilution buffer for use: 1X PBS, 3% BSA (IgG-free). The antibody is unlabeled, therefore detection may be accomplished with any appropriately labeled anti-rabbit secondary antibody. Alternatively, plates may be coated with a 1:1000 dilution of antibody in 0.05M Na2CO3, pH 9.6.
Optimal dilutions to be determined by the researcher.

Nuclease Activity:
No detectable nuclease activity when incubated with single or double stranded DNA for 16 hours at 37C.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceE. coliRabbit
Full length E. coli MutS
Purified by Protein A affinity chromatography.
Supplied as a liquid in 78mM sodium chloride, 6mM K2HPO4, pH 7.4, 1.3mM potassium chloride, 50% glycerol.
Recognizes E. coli MutS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.