Technical Data
M9600-03B
Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian))
Description:
Transcription factor Myb is expressed in hematopoietic cells and immature gastroepithelial cells.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:12.5-1:25 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat and Ls174T cells. Testis.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a5G83Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Recombinant v-myb protein. Cellular Localization: Cytoplasmic and nuclear
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide.
Specificity:
Recognizes human Myb at ~80kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.