		Technical Data
M9600-03B	Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian))

Description:
Transcription factor Myb is expressed in hematopoietic cells and immature gastroepithelial cells.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:12.5-1:25 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat and Ls174T cells. Testis.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	5G83	Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Recombinant v-myb protein. Cellular Localization: Cytoplasmic and nuclear

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide.

Specificity:

Recognizes human Myb at ~80kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.