Technical Data
Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian))
Transcription factor Myb is expressed in hematopoietic cells and immature gastroepithelial cells.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:12.5-1:25 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat and Ls174T cells. Testis.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a5G83Affinity Purified
250ul-20CBlue IceHumanMouse
Recombinant v-myb protein. Cellular Localization: Cytoplasmic and nuclear
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide.
Recognizes human Myb at ~80kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.