Technical Data
M9699-58B
Mycobacterium tuberculosis ESAT6 (6kD Early Secretory Antigen of T Cells)
Description:
The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9B87Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to ESAT6 M. tuberculosis H37RV
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the ESAT6 antigen of Mycobacterium tuberculosis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorensen, A.L., et al., Infect. Immun. 63: 1710-1717 (1995). 2. Ravn, P., et al., J. Infect. Dis. 179: 637-645 (1999). 3. Klausen, et al., Scand. J. Immunol. 40: 345-349 (1994). 4. Harboe, M., et al., Infect. Immun. 66: 717-723 (1998). 5. Harboe, M., et al., Infect. Immun. 64: 16-22 (1996).