Technical Data
M9755-10C
MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)
Description:
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot (ECL) (4): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
Immunogen:
Human MyD88 synthetic peptide (residues 279-296). This sequence is identical to mouse MyD88 over these residues.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
This immunoaffinity purified antibody Detects a ~35kD protein, corresponding to the apparent molecular mass of MyD88 on SDSPAGE immunoblots, in samples from human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonnert, T.P., Garka, K.E., Parnet, P., Sonoda, G., Testa, J.R. and Sims, J.E. (1997) FEBS
Lett. 402: 81-84.
2. Liebermann, D.A. and Hoffman, B. (1998) Oncogene 17: 3319-3329.
3. Kawai, T., Adachi, O., Ogawa, T., Takeda, K. and Akira, S. (1999) Immunity 11: 115-122.
4. Kobayashi, K., Hernandez, L.D., Galan, J.E., Janeway, C.A. Jr., Medzhitov, R., Flavell,
R.A. (2002) Cell 110(2): 191-202.