		Technical Data
M9755-10R	MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)

Description:
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappa B and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon) and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of I-kappa B that normally maintains NF--kappa B inactivity by sequestering it in the cytoplasm. MyD88 was originally isolated as a myeloid differentiation primary response gene that is rapidly induced upon IL-6 stimulated differentiation of M1 myeloleukemic cells into macrophages (4-6). It contains an amino-terminal death domain separated from a carboxyl-terminal TIR domain and functions as an adaptor in TLR/IL-1 receptor signaling (7). The death domain of MyD88 mediates interactions with the IRAK complex triggering a signaling cascade that includes the activation of NF--kappa B (8,9).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	10E41

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to residues surrounding Cys233 of human MyD88 protein (KLH- coupled).

Purity:

Form

Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and 0.02% sodium azide.

Specificity:

Recognizes human MyD88. Species crossreactivity: mouse, rat. Sequence Homology: monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Akira, S. (2003) J Biol Chem 278, 38105-8, 2. Beutler, B. (2004) Nature 430, 257-63, 3. Dunne, A. and O’Neill, L.A. (2003) Sci STKE 2003, re3, 4. Harroch, S. et al. (1995) Nucleic Acids Res 23, 3539-46, 5. Hardiman, G. et al. (1996) Oncogene 13, 2467-75, 6. Bonnert, T.P. et al. (1997) FEBS Lett 402, 81-4, 7. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8, 8. Wesche, H. et al. (1997) Immunity 7, 837-47, 9. Muzio, M. et al. (1997) Science 278, 1612-5.