Technical Data
MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappa B and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon) and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of I-kappa B that normally maintains NF--kappa B inactivity by sequestering it in the cytoplasm.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues surrounding Cys233 of human MyD88 protein.
Supplied in liquid 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total human MyD88. Species crossreactivity: mouse, rat, monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Akira, S. (2003) J Biol Chem 278, 38105-8, 2. Beutler, B. (2004) Nature 430, 257-63, 3. Dunne, A. and ONeill, L.A. (2003) Sci STKE 2003, re3, 4. Harroch, S. et al. (1995) Nucleic Acids Res 23, 3539-46, 5. Hardiman, G. et al. (1996) Oncogene 13, 2467-75, 6. Bonnert, T.P. et al. (1997) FEBS Lett 402, 81-4, 7. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8, 8. Wesche, H. et al. (1997) Immunity 7, 837-47, 9. Muzio, M. et al. (1997) Science 278, 1612-5.