Technical Data
M9758-03
Myelin Basic Protein (MBP)
Description:
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed and paraffin embedded): 1:100 by PAP or ABC. Pretreatment of tissue with proteolytic enzyme is not required.
Western Blot: 1:1500 in TTBS, overnight at 4C.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Human myelin basic protein from brain.
Purity:
Purified immunoglobulin fraction from ammonium sulfate fraction.
Form
Supplied as a liquid in 0.05M Tris HCl, pH 7.6, 15mM sodium azide before the addition of glycerol to 40%.
Specificity:
Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McTigue, D.M., et al., J. Neuroscience 18: 5354-5365 (1998).