Technical Data
M9758-03T
Myelin Basic Protein, aa129-138 (MBP)
Description:
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (frozen): 1:10
ELISA: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H2754Supernatant
SizeStorageShippingSourceHost
2ml-20CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Bovine Myelin Basic Protein (MBP)
Purity:
Supernatant
Form
Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Specificity:
Recognizes bovine Myelin Basic Protein. Reactive with an epitope in the aa129-138 region of the human MBP molecule. Species Crossreactivity: human, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Groome, N.P.et al. (1985) Neurochem. Int. 7(2): 309-317.2. Martenson, R. E. (1984) Experimental allergic encephalomyelitis. A useful model for multiple sclerosis. In Alvord, E. C. et al. (Eds).Wiley, New York, pp273-289.3. Groome, N. P. et al. (1986)J Neuroimmunol. 12: 253-264. 4. Marignier, R. et al. (2010) Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury.Brain. Aug 5. [Epub ahead of print] 5. Brunner, C. et al. (1989) Differential ultrastructural localization of myelin basic protein, myelin/oligodendroglial glycoprotein, and 2',3'-cyclic nucleotide 3'-phosphodiesterase in the CNS ofadult rats.J Neurochem. 52: 296-304.6. Jatana, M. et al. (2006) Combination of systemic hypothermia and N-acetylcysteine attenuates hypoxic-ischemic brain injury in neonatal rats. Pediatr Res. 59: 684-9.7. Chujor, C.S. et al. (1991) Serum antibodies against peripheral nervous system antigens in leprosy. Int J Lepr Other Mycobact Dis. 59: 590-7.