Technical Data
M9758-04
Myelin Basic Protein, Degraded (MBP)
Description:
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles (3). MBP serves as a marker for oligodendrocytes and Schwann cells (3). MBP is also a marker for malignant schwannomas (4) and appears to play a significant role in the etiology of multiple sclerosis (5–7) and experimental autoimmune encephalomyelitis (8).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:2000. Immunostains myelin basic protein of abnormal appearing oligodendrocytic processes and cell bodies in demyelinating areas. Recognizes only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerve. It also stains discrete white matter in brain of multiple system atrophy.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa69-86 of the guinea pig Myelin Basic Protein, Degraded.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 50ul of sterile ddH2O. Preservative free.
Specificity:
Recognizes Myelin Basic Protein in demyelinated nerve tissues. High specificity for lesioned rat spinal cord. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Matsuo, A., et al., Am. J. Pathol. (1997) 150(4):1253–1266. 2. Day, E. D. and Potter, N. T (1986) J. Neuroimmunol. 10, 289. 3 Hashim, G. A. (1978) Immunol. Rev. 39, 60. 4. Golo, M. (1987) Histochemistry 87, 201. 5. Mogollon, R, et al. (1984) Cancer 53, 1190. 6. Whitaker, J. N. (1984) Ann. N.Y. Acad. Sci. 436, 140. 7. Eidinoff, H. (1988) Med. Hypotheses 26, 103. 8. tar Meulen, V. (1988) Ann. N.Y. Acad. Sci. 540, 202. 9. Kerlero de Rosbo, N. et al. (1985) J. Neuroimmunol. 9, 349. 10. Sires, L. R. et al. (1981) Science 214, 87.