Technical Data
MOG (Myelin-oligodendrocyte Glycoprotein)
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). MOG protein constitutes only 0.01-0.5% of the CNS myelin proteins, has a mass of 27kD, and is located in the outermost layer of myelin sheath. Mouse MOG is able to induce autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination. Auto- antibody response to mouse MOG has been observed in induced experimental autoimmune encephalomyelitis (EAE) in C57/BL6, 129/Sv, B6, and SJL mice.

Suitable for use in Immunoprecipitation, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug/10e7 cell extract
Western Blot: 1:1000
Immunohistochemistry: 1:50-1:200
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceMouse
Not determined
Recombinant corresponding to myelin oligodendrocyte glycoprotein.
Supplied as a liquid in in 40mM MOPS, pH7.5, 50% glycerol, 0.09% sodium azide, 0.1% BSA.
Recognizes MOG. Species Crossreactivity: mouse, human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Linares, D. et al. Prot. Expr. and Purif. 34, 249 (2004). 2. Bettadapura, J. et al. J. Neurochem. 70, 1593 (1998). 3. Oliver, AR. et al J. Immunol. 171, 462 (2003). 4. Von Budingen, H-C. et al. J. Clin. Imm.21, 155 (2001). 5. Lyons, J-A. Eur. J. Immunol. 29, 3432 (1999). 6. Von Budingen, H-C. Eur. J. Immunol. 34, 2072 (2003).