Technical Data
M9850-11
Myosin, Heavy Chain
Description:
Myosin is a 480kD protein known to interact with actin in muscle and in non muscle cells. It contains two identical heavy chains (200kD each) and four light chains (1526kD). Myosin molecules consist of two major regions: tails (rod) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type. Skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slow-twitch) and type II (fast-twitch).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 dilution detects human cardiac myosin in RIPA homogenate of human heart. Human heart homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with M9850-11. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system..
Immunohistochemistry: Detects myosin in human ventricle sections.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG5H65Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Purified human cardiac myosin heavy chain.
Purity:
Ascites
Form
Supplied as a liquid, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes human myosin, cardiac, Mr 220kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Simeonova, P.P., et al., Clin. Chim. Acta., 201:207-222, 1991.2. Sugiura, S. and H. Yamashita, Jpn. J. Physiol., 48: 173-179, 1998.3. Morano, I., J. Mol. Med., 77: 544-555, 1999.