Technical Data
M9850-11C
Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po
Description:
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100-1:2000
Immunohistochemistry: 1:10-1:2000
Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites
Immunohistochemistry(Paraffin): 1:400
Western Blot: 1:100-1:2000. Detects a band at ~223kD
Immunocytochemistry (IF): 1:10-1:2000
Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.
Optimal dilutions to be determined by the researcher.

Affinity Constants:
Human ventricular myosin heavy chains: 3.33 x10e8/M Human atrial myosin heavy chains: 1.48 x10e8/M
Human skeletal muscle myosin: 1.06 x10e8/M
Canine cardiac myosin heavy chains: 2.06 x10e8/M

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8L880Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified full length native human Myosin Cardiac, Heavy Chain.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes human myosin alpha and beta heavy chains. Reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. (Myosin heavy chain is specific for cardiomyocytes.). It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction. Species Crossreactivity: bovine, mouse, rabbit, rat, canine and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.