Technical Data
Myostatin, Recombinant, Human (E. coli) (Lyophilized) (Growth Differentiation Factor 8, MSTN, gdf8)
Molecular Biology Storage: -20CShipping: Blue Ice
Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. The myostatin pathway is conserved and regulates muscle mass in a number of animal species ranging from fish to humans. Inhibition of myostatin using a variety of therapeutic approaches can increase muscle mass in a number of animal models of human disease, including muscular dystrophy.

Recombinant Human Myostatin, also called GDF-8, produced in E. coli, is a homodimer, non-glycosylated polypeptide chain containing 2x109 amino acids and having a total molecular mass of 24.814kD.

AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.

Biological Activity:
Recombinant Human Myostatin is fully biologically active when compared to standard. The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is ~ 20ng/ml.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.

Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 20mM acetic acid, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% by RP-HPLC, Anion-exchange FPLC, Analysis by reducing and SDS-PAGE.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder from a concentrated (1mg/ml) solution containing no additives. Reconstitute with sterile 20mM acetic acid to a concentation of 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA. Lyophilized myostatin is poorly soluble at neutral pH.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Biochem. Biophys. Res. Commun. 340(2): 675-680 (2006). 2. Commun. Agric. Appl. Biol. Sci. 70(2): 37-41 (2005). 3. Anim. Genet. 36(6): 521 (2005). 4. Dev. Dyn. 234(4): 1064 (2005). 5. DNA Seq. 16(6): 412-417 (2005). 6. Gerard, Y., et al., Infection 23: 310311 (1995). 7. Assicot, M., et al., Lancet 341: 515518 (1993).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.