Myostatin, Recombinant, Human (E. coli) (Lyophilized) (Growth Differentiation Factor 8, MSTN, gdf8)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. The myostatin pathway is conserved and regulates muscle mass in a number of animal species ranging from fish to humans. Inhibition of myostatin using a variety of therapeutic approaches can increase muscle mass in a number of animal models of human disease, including muscular dystrophy.
Recombinant Human Myostatin, also called GDF-8, produced in E. coli, is a homodimer, non-glycosylated polypeptide chain containing 2x109 amino acids and having a total molecular mass of 24.814kD.
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.
Recombinant Human Myostatin is fully biologically active when compared to standard. The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is ~ 20ng/ml.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.
Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.
Optimal dilutions to be determined by researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile 20mM acetic acid, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: ~95% by RP-HPLC, Anion-exchange FPLC, Analysis by reducing and SDS-PAGE.
Form: Supplied as a lyophilized powder from a concentrated (1mg/ml) solution containing no additives. Reconstitute with sterile 20mM acetic acid to a concentation of 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA. Lyophilized myostatin is poorly soluble at neutral pH.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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