Technical Data
M9931-25A
MYRIP (SLAC2C, OTTHUMP00000209206, MGC130035, MGC130034, FLJ44025, synaptotagmin-like protein homologue lacking C2 domains-c, Slp homologue lacking C2 domains, rab effector MYRIP, DKFZp586F1018, exophilin 8, exophilin, SLAC2-C, MyRIP, myosin VIIA and Rab
Description:
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64000
Western Blot: 0.3-1ug/ml. ~90kD band observed in human brain amygdala lysates (calculated MW of 95.7kDaaccording to NP_056275.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-KDLMEPALESAVMY from the C Terminus of human MYRIP (NP_056275.2).
Purity:
Purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form
Supplied as a liquid in Tris saline, pH7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human MYRIP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. El-Amraoui A, Schonn JS, Kussel-Andermann P, Blanchard S, Desnos C, Henry JP, Wolfrum U, Darchen F, Petit C., 2. MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes.
EMBO Rep. 2002 May;3(5):463-70.