Technical Data
N0020-01R
NRAMP2 (Natural Resistance-associated Macrophage Protein 2, NRAMP 2, Divalent Cation Transporter 1, Divalent Metal Transporter 1, DMT-1, DCT1, DMT1, SLC11A2, OK/SW-cl.20)
Description:
Solute carrier family 11, member 2 (SLCA2) also known as divalent metal ion transporter 1 (DMT1) imports Fe2+ via a proton-coupled, membrane potential dependent manner. It can also transport a variety of other divalent metal cations including Mn2+, Co2+, Cu2+, and Zn2+ but iron appears to be its most important physiological substrate. SLC11A2 has been demonstrated on the apical membrane of duodenal enterocytes, transferrin cycle endosomes in erythroid precursors, hepatocytes where it has been postulated to be involved in non-transferrin-bound iron uptake, and placenta where it has been implicated in transfer of iron from mother to fetus.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k4C6Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant corresponding to aa1-66 from human SLC11A2 with GST tag. MW of the GST tag alone is 26kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human SLC11A2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.