Technical Data
N0021
Naked2 (Nkd2)
Description:
Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif; however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/ -catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/ -catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF- to the basolateral plasma membrane of polarized epithelial cells (6).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
IImmunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG8A19
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseRabbit
Concentration:
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Gly47 of mouse Naked2.
Purity:
As reported
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/mlBSA, 50% glycerol, 0.02% sodium azide.
Specificity:
Detects transfected levels of mouse Naked2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Zeng, W. et al. (2000) Nature 403, 789–795.
(2) Wharton, K.A. et al. (2001) Dev. Biol. 234, 93–106.
(3) Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019–49026.
(4) Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802–3807.
(5) Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973–14978.
(6) Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571–5576.