Technical Data
NAPE-PLD (N-acyl Phosphatidylethanolamine Phospholipase D, N-Acylphosphatidylethanolamine-Hydrolyzing Phospholipase D, NAPE-hydrolyzing Phospholipase D, NAPEPLD, C7orf18, FMP30, Mbldc1)
N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.

Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:1000-1:5000
Western Blot: 1:2500-1:5000
Immunohistochemistry: 1:1000-1:5000
Immunocytochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Positive Controls:
Mouse Brain total protein

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceMouseRabbit
Not Determined.
Synthetic peptide corresponding to an internal portion of the mouse protein (within residues 1-100). [Swiss-Prot# Q8BH82].
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes mouse NAPE-PLD at ~50kD. Species Crossreactivity: Human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, J. et al. Neuropharmacology 54(1):8-15 (2008) 2. Fu, J. et al. Am J Physiol Regul Integr Comp Physiol. 295(1):R45-50 (2008) 3. Egertova, M. et al. J Comp Neurol. 506(4):604-15 (2008) 4. Okamoto, Y. et al. J. Bio. Chem. 279(7):5298-5305 (2004).