Technical Data
N0536-07A
NBC1 (Na, HCO3 Cotransporter 1, pNBC)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. A great majority of HCO3-reabsorption occurs via trans-cellular coupling of the luminal Na-H+-exchanger 3 and Na+-H+-ATPase with the basolateral Na+-HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney. NBC1 is 30-35% identical with AEs. It is strongly expressed in the kidney and pancreas. The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1. Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC. pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml; using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000; Using 50-100ng of control peptide/well. See N0536-08.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
As reported
Immunogen:
20aa peptide from rat NBC1, conjugated to KLH. Epitope Location: ~C-terminus, Cytoplasmic domain. No significant sequence homology was found with NBC2 or NBC3.
Purity:
Serum
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes rat NBC1. Antibodies will recognize both the kidney and pancreatic/heart isoforms of NBC1. Species Sequence Homology: Mouse - 100%; Human - 85%; Frog, rabbit - 80%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Romero MF (1998) AJP 274, F425-F432 Burnham CE (1998)
Burnham CE (1997) JBC 272, 19111-19114
Abuladze N (1998) JBC 273; 17689-17695
Solemani M (2000) Kidney Intl. 57, 371-384 (review)
Romero MF & Boron WF (1999) Ann. Rev. Physiol. 61, 699-723 (review)
Velic A. 2004, Am. Soc. Nephrol., 15: 967 - 977
Robey RB 2002, Journal of Membrane Biology