Technical Data
N0536-10
NBC1 (Na, HCO3 Cotransporter 1, pNBC)
Description:
Bicarbonate, along with CO2, is the major pH buffer of biological fluids. The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases. Several organs specialize in the mobilization of HCO3-. The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions. The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma. To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O. A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC). Several related proteins constitute the emerging NBC family (NBC1-3) of membrane cotransporters that are found in a variety of epithelial and non-epithelial tissues, and may be tissue specific. Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
NBC1 (human, rat, mouse 1035 aa, also called NBC-1A/1B, hkNBC, rkNBC, pNBC, hhNBC, SLC4A4) was initially cloned from human Kidney. NBC1 is 30-35% identical with AEs. It is strongly expressed in the kidney and pancreas. The rat kidney NBC1 (rkNBC1; 1035 aa) is 86% identical to hkNBC1. Pancreatic NBC (pNBC/hhNBC/SLC4A5, human 1079 aa) has a unique n-terminal 85 aa sequence replacing the 41-aa in kNBC. pNBC is also more widely expressed (pancreas, thyroid, heart, and brain).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL)
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide: N0536-15

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic 19-aa peptide sequence within the cytoplasmic, N-terminus of rat NBC1 (KLH).
Purity:
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat NBC1.This region is unique to the kidney isoforms and it is not found in pancreatic isoform, pNBC/PSLC4A5. No significant sequence homology of was found with NBC2 or NBC3. Species sequence homology: mouse 100%, human and rabbit 94% NBC1/PSLC4A4/kNBC
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Romero MF et al (1998) Am. J. Physiol. 274, F425-F432 Burnham CE et al (1998) gene accession # AF027362; Burnham CE et al (1997) J. Biol. Chem. 272, 19111-19114; Abuladze N et al (1998) J. Biol. Chem. 273; 17689-17695; Giffard RG et al (2000) J. Neurochem. In press; Solemani M & Burnham CE et al (2000) Kidney Intl. 57, 371-384 (review); Romero MF & Boron WF et al (1999) Ann. Rev. Physiol. 61, 699-723 (review)