		Technical Data
N0570-12	NDC80 Homolog, Kinetochore Complex Component (HEC, HEC1, KNTC2, hsNDC80, TID3)

Description:
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8C229	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C/-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Partial sequence of recombinant full-length protein to human NDC80 Homolog, Kinetochore Complex Component

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

Specificity:

Recognizes human NDC80 Homolog, Kinetochore Complex Component.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.