		Technical Data
N0570-12B	NDC80 Homolog, Kinetochore Complex Component (HEC, HEC1, Highly Expressed in Cancer, Highly Expressed in Cancer Rich in Leucine Heptad Repeats, HsHec1, hsNDC80, Kinetochore Associated 2, KNTC2, Kinetochore Protein Hec1, NDC80, Retinoblastoma Associated Pr

Description:
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Applications:
Suitable for use in ELISA (peptide) and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide: YEKKATLIKSSEE representing the C-Terminus of the human protein (residues 630-642), according to NP_006092.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

Specificity:

Species Crossreactivity: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Chen Y et al. HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Mol Cell Biol 17:6049-56 (1997).