Technical Data
Neuroligin 2 (NLGN2, KIAA1366)
NLGN2 (Neuroligin 2) is a 105kD member of the type-B carboxylesterase family of enzymes. It is expressed at GABAergic synapses, and is assumed to play both an organizing and regulatory role in inhibitory synapse signaling. It is suggested to bind to beta -neurexin-1, 2 and 3 as a dimer in trans, and to interact cytoplasmically with PDZ-domain containing proteins. Mature human NLGN2 is an 821aa type I transmembrane (TM) glycoprotein. It contains a nonfunctional type-B lipase domain (aa40-601), a TM segment (aa678-698), and a cytoplasmic tail with a PZD-binding region (aa785-835). There are potential splice forms. Individually, or in combination, there is an alternate start site at Met512, a deletion of aa153-169, and a 99aa substitution for aa 806-835. Over aa1-677, human NLGN2 shares 98% aa identity with mouse NLGN2.

Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Neuroligin 2 is ~0.5ng/well.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
~0.2mg/ml (after reconstitution)
Recombinant protein corresponding to aa15-660 at the extracellular domain of human Neuroligin 2 expressed in NS0 cells (Accession# EAW90197).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human and rat Neuroligin 2 extracellular domain. Species Crossreactivity: recombinant human NGLN1, NGLN3, NGLN4 <5%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.