Technical Data
N2025-02
NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)
Description:
Na+/ H+ exchanger regulatory factor (NHERF1) is a PDZ domain containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements and cytoplasmic signaling proteins. A 358aa mainly expressed in kidney, liver and pancrease by NHERF gene (chrm 17). And is represented in another isoform, NHERF2. NHERF1 protein contains 2 tandem PDZ domains of approximately 90aa and a C-terminal sequence that binds several members of the ERM (ezrin-radixin-moesin) family of membrane cytoskeletal adapters. The protein plays an important role in regulation of NHE3, turnover of G-protein coupled receptors, platelet derived growth factor receptor and ion transporters such as CFTR, Na/Pi cotransporter, NaHCO3 cotransporter and Trp channels. NHERF1 overexpression in cancers and mutations in NHERF1 targets, Merlin, the product of tumor suppressor gene, also expressed in 2 isoforms, Merlin is involved in pathogenesis of benign tumors of the human nervous system, Merlin is named for its striking similarity with ERM family which thought to link cytoskeletal components with proteins in the cell membrane, It has a binding partner called Syntenin, which is an adapter protein that couples transmembrane proteoglycans to cytoskeletal components.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA:0.5-1ug/ml. Coat ELISA plates at 1ug/ml. with N2025-02A Control peptide.
Western Blot: 1-10ug/ml using ECL technique.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa from the C-terminal, cytoplasmic domain of Human NHERF1 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Specificity:
Recognizes human NHERF1. Species Sequence Homology (92%): Mouse, rabbit, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Edward J. Weinman et al (2001) JBC 276, 42339-42346; Junqi He et al (2001) JBC 276, 41559-41565; Anita Murthy et al (1997) JBC 273, 1273-1276; Mehrdad Jannatipour et al (2001) JBC 276, 33093-33100; Edward J. Weinman et al (2001) Jour. Of Clinical Investigation, 108, 185-186.