Technical Data
Nerve Growth Factor (NGF) (High Molecular Weight Complex)
Molecular Biology Storage: -20°C/-70°CShipping: Blue Ice
NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.

Biological Assay:
The neurotrophic activity is in the range of 0.5-50ng/ml using rat PC12 cells cultured for 7-14 days. The recommended Concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium.

Storage and Stability:
Lyophilized powder can be stored at -20ºC for 6 months. After reconstitution, store at -70ºC for 4-6 weeks. Avoid repeated freeze/thaw cycles to prevent loss of activity.
Source: Mouse submaxillary gland
Purity: ~97% pure as determined by SDS-PAGE
Concentration: As reported
Form: Supplied as a lyophilized powder. Reconstitute with sterile DMEM at a concentration of 10ug/mL and stored at-70°C. Medium with mNGF cannot be reused due to mNGF degradation.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. In Darling, T. and Shooter E.M. (1984), “Methods for preparation and assay of nerve growth factor.” Cell Culture Methods for Molecular and Cellular Biology (Eds. D.W. Barnes, D.A. Sinbasku and G.H. Sato) pp 79–83, Alan R. Liss, New York. 2. Varon, S., et al., Biochem. (1967) 6:2202.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.