		Technical Data
N2060-05A	Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Description:
Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug labels 10e6 cells for intracellular staining. Validated using A172 human glioblastoma cell line fixed with parafomaldehyde and permeabilized with saponin.
Immunocytochemistry: 8-25ug/ml. Validated using immersion fixed human neural progenitor cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	3k1	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

NSO cells transfected with aa618-1618 of human Nestin.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Specificity:

Recognizes human Nestin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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