Technical Data
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in the neural differentiation pathway. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors.

Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug labels 10e6 cells for intracellular staining. Validated using A172 human glioblastoma cell line fixed with parafomaldehyde and permeabilized with saponin.
Immunocytochemistry: 8-25ug/ml. Validated using immersion fixed human neural progenitor cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG13k1Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
NS0 cells transfected with human Nestin. Uni/Swiss Prot#: P48681
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human Nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hockfield, S., et al., J. Neurosci. 5: 3310-3328 (1985). 2. Lendahl, U., et al., Cell 60: 585-595 (1990). 3. Frederiksen, K., et al., J. Neurosci. 8: 1144-1151 (1988). 4. Tohyama, T., et al., Lab. Invest. 66: 303-313 (1992). 5. Uchida, N., et al., PNAS USA 97: 14,720-14,725 (2000). 6. Frederiksen, K., et al., Neuron 1: 439-448 (1988). 7. Cattaneo, C., et al., Nature 347: 762-765 (1990). 8. Reynolds, B.A., et al., Science 255: 1707-1739 (1992). 9. Rietze, R.L., et al., Nature 412: 736-739 (2001). 10. Carpenter, M.K., et al., Exp. Neurol. 172: 383-397 (2001). 11. Zulewski, H., et al., Diabetes 50: 521-533 (2001). 12. Lumelsky, N., et al., Science 292: 1389-1394 (2001). 13. Lechner, A., et al., Biochem. Biophys. Res. Commun. 293: 670-674 (2002). 14. Shih, C.C., et al., Blood 98: 2412-2422 (2001).