Technical Data
N2060-05B
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Description:
Nestin is a class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.
Immunohistochemistry (frozen/paraffin): 1:100-1:200. Using a fluorescein or rhodamine conjugated anti-mouse secondary antibody.
Western Blot: 1:1000-1:5000 using chemiluminescent detection.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Nestin transfected NS0 mouse myeloma cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15C93Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to 150aa fragment from human nestin conjugated to glutathione S-transferase.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human nestin protein. Does not crossreact with rodent Nestin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Messam, C., J. Hou, and E.O. Major. Co-expression of nestin in neural and glial cells in the developing human CNS defined by a human specific anti-nestin antibody. Exp Neuro; Feb;161(2):585-96; 2000. 2. Messam C, Hou J, Berman JW, and Major EO. Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells. Developmental Brain Research. 134:87-92, 2002. 3. Attardo et al., Cerebral Cortex (2010) 20:304-314.
General references: 1. Hockfield, S., et al., J. Neurosci. 5:3310-3328 (1985). 2. Lendahl, U., et al., Cell 60:585-595 (1990). 3. Frederiksen, K., et al., J. Neurosci. 8:1144-1151 (1988). 4. Tohyama, T., et al., Lab. Invest. 66:303-313 (1992). 5. Uchida, N., et al., PNAS USA 97:14,720-14,725 (2000). 6. Frederiksen, K., et al., Neuron 1:439-448 (1988). 7. Cattaneo, C., et al., Nature 347:762-765 (1990). 8. Reynolds, B.A., et al., Science 255:1707-1739 (1992). 9. Rietze, R.L., et al., Nature 412:736-739 (2001). 10. Carpenter, M.K., et al., Exp. Neurol. 172:383-397 (2001). 11. Zulewski, H., et al., Diabetes 50:521-533 (2001). 12. Lumelsky, N., et al., Science 292:1389-1394 (2001). 13. Lechner, A., et al., Biochem. Biophys. Res. Commun. 293:670-674 (2002). 14. Shih, C.C., et al., Blood 98: 2412-2422 (2001).