		Technical Data
N2160-12A	NF-H (Neurofilament heavy polypeptide, Neurofilament triplet H protein, 200kD, NEFH, KIAA0845, NFH)

Description:
Neurofilaments contain three intermediate filament proteins: L, M and H, which are involved in the maintenance of the neuronal caliber. NF-H has an important function in mature axons. NF-H is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Defects in NF-H are a cause of amyotrophic lateral sclerosis (ALS) which is a neurodegenerative disorder affecting upper and lower motor neurons and results in fatal paralysis.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	9G402	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Partial recombinant human NF-H protein (263-364) with a GST tag.

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

Specificity:

Recognizes human NF-H.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.