Technical Data
N2175-04
Neuron Specific Enolase (NSE)
Description:
Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD), and gamma (46kD). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100-1:300
Optimal dilution must be determined by the user.

Recommended Pair:
Capture: N2175-04
Detection: N2175-06

Affinity Constant:
1x10e-8 - 1x10e-9 L/mole

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a1.B.648Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Purified neuron-specific enolase from human brain.
Purity:
Protein by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes gamma enolase subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.