Technical Data
N2175-17
Neuron Specific Enolase (NSE)
Description:
Neuron specific enolase (NSE) is the neuronal form of the enolase glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. NSE has a molecular weight of 45kD, and is expressed in normal pituitary, pinealocytes, neuroendocrine cells of the lung, thyroid, parafollicular cells, adrenal medulla, Langerhans islet cells, Merkel cells of the skin, melanocytes, normal striated muscle, and hepatocytes. NSE is a useful marker for neuroblastoma, small cell lung carcinoma, and neuroendocrine cancers.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin fixed paraffin embedded tissue sections. No pretreatment or epitope retrieval required.
Optimal dilutions to be determined by the researcher.

Positive Control Tissue:
Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k3G208Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Bovine brain. Expected Staining Pattern: Cytoplasmic.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes the gamma subunits of human NSE (neuronal origin) and does not react with the alpha subunits (glial origin). Some crossreactivity with the beta subunits of NSE may be observed in muscle. In normal tissues, it has been shown to stain pancreas, brain, colon and stomach.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rode J, et al. Diagnostic Histopathol 5:205, 1982. 2. Simpson S, et al. Cancer 54:1364, 1984.