Technical Data
N2176-60B
Neuropeptide Y (NPY, Neuropeptide Tyrosine, Neuropeptide Y Precursor, PYY4, Y Neuropeptide)
Description:
Neuropeptide Y (NPY), a 36 amino acid peptide amide, is a major regulatory neuropeptide widely distributed in the mammalian central (CNS) and peripheral nervous systems (PNS). NPY belongs to the pancreatic polypeptide family of peptides which are characterized by a common tertiary structure (1,2). Within this family, an intestinal peptide hormone, peptide YY (PYY), is most closely related to NPY. In the CNS, NPY is involved in regulation of blood pressure, memory processing, circadian rhythm, and stimulation of food intake (2). In the PNS, NPY has potent vasoconstrictor activity and acts as a neurotransmitter/neuromodulator of sympathetic neurons and adrenal glands (3,4). NPY is one of the most abundant peptides found in the CNS, widely distributed in the brain. High levels of NPY are present in the cerebral cortex, amygdaloid nuclei, hippocampal formation, and hypothalamus (5-7). In the PNS, NPY is found mainly in sympathetic neurons that innervate vascular smooth muscle, heart, and urogenital tract (3,8,9) The biological actions of NPY in the brain and periphery are mediated by at least two different NPY receptors, designated Y1 and Y2 receptor subtypes (10-11).

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Indirect ELISA: 1:5000-1:10,000 using peptide coating at 0.5-1ug/well in 0.05M carbonate/bicarbonate buffer, pH 9.6 and pNPP as substrate
Immunohistochemistry (Frozen): 1:8000-1:16,000 using 4% paraformaldehyde perfusion-fixed sections of rat brain
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IcePorcineRabbit
Concentration:
Not Determined
Immunogen:
Synthetic porcine Neuropeptide Y (KLH) (GeneID 397304). The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY.
Purity:
Serum
Form
Supplied as a liquid, 15mM sodium azide.
Specificity:
Recognizes porcine Neuropeptide Y. Crossreacts with human Peptide YY (PYY). Does not crossreact with Substance P, Neurokinin A, Neurokinin B, Calcitonin, Somatostatin or BSA. Species Crossreactivity: human, rat and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tatemoto, K., et al., Nature, 296, 659-660 (1982). 2. Emson, P., and DeQuidt, M., Trends Neurosci., 7, 31-35 (1984). 3. Lundberg, J. M., et al., Acta Physiol. Scand., 116, 477-480 (1982). 4. Edvinsson, L., et al., Trends Pharmacol. Sci., 8, 231-235 (1987). 5. deQuidt, M. E., and Emson, P., Neurosci., 18, 545-618 (1986). 6. Higuchi, H., et al., J. Biol. Chem., 263, 6288-6295 (1988). 7. Chan-Palay, V., et al., J. Comp. Neurol., 238, 382- 389 (1985). 8. Schalling, M., et al., Neurosci., 41, 753-766 (1991). 9. Sundler, F., et al., Cell Tissue Res., 230, 487-493 (1983). 10. Michel, M. C., Trends Pharmacol. Sci., 12, 389-394 (1991). 11. Quirion, R., et al., Ann. NY Acad. Sci., 611, 58-72 (1990).