Technical Data
Neurturin, Blocking Peptide (NTN)
Molecular Biology Storage: -20CShipping: Blue Ice
Peptide corresponding to amino acids 178 to 193 of human neurturin (1).

Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin (1, 2). Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRa (3,4). The cDNAs encoding NTNRa from human, rat, chicken, and mouse have been cloned recently (3-8) and termed GDNFRb, Ret ligand 2 (RETL2) or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently (9). NTN binds to and forms a complex with GFRa-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 24 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Synthetic peptide
Purity: Purified 60-70%
Concentration: 0.2mg/ml
Form: Supplied as a lyophilized powder.

Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470 2. Heuckeroth RO, Kotzbauer P, Copeland NG, et al. Neurturin, a novel neurotrophic factor, is localized to mouse chromosome 17 and human chromosome 19p13.3. Genomics 1997;44(1):137-40 3. Klein RD, Sherman D, Ho WH, et al. GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997;387:717-721 4. Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724 5. Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-802 6. Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci USA 1997;94:6238-6243 7. Suvanto P, Wartiovaara K, Lindahl M, et al. Cloning, mRNA distribution and chromosomal localisation of the gene for glial cell line-derived neurotrophic factor receptor beta, a homologue to GDNFR-alpha. Hum Mol Genet 1997;6:1267-1273 8. Wang CY, Ni J, Jiang H, et al. Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B: a novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. Neuroscience 1998;83(1):7-14 9. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFRa Neuron 1997;19:485 (RD0500)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.