Technical Data
N2254-01
Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (Biotin)
Description:
Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G is a GPI- anchored protein and a good marker of peripheral neutrophils. Although predominantly present on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells and T cell subsets. N2254-01 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has an inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

Applications:
Suitable for use in ELISA, Flow Cytometry, Functional Studies and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50 (10ug/ml) labels 5x10e5 cells (4)
Immunohistochemistry (Frozen): 1:50 acetone fixation is recommended. (7)
Immunohistochemistry (Paraffin): 1:50 blocking with 20% normal rabbit serum. (5)
Functional Studies (Remove sodium azide): Neutrophil depletion. Mice were tested with NIMP-R14 given intraperitoneally at a dose of 1mg, 6 hours before injection. (6)
Optimal dilutions to be determined by the researcher.

Recommended Positive Control: Mouse neutrophils

Recommended Negative Control: Mouse Thymocytes

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b6A608Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Purified BALB/c mouse neutrophils
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
Specificity:
Recognizes mouse Ly-6G and Ly-6C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lopez, A et al; Brit J Haematology 1984, 57: 489 2. McLaren, D et al; Parasite Immunol 1987, 9: 667 3. Folkard, S et al; Parasitology 1996, 112: 323 4. Van Lent, P et al; J Leukoc Biol 1997, 61: 267 5. Lubberts, E et al; Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction. J Immunol 1999, 163: 4546 6. Tacchini-Cottier, F et al; J Immunol 2000, 165: 628 7. Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883 8. Nagendra, S et al; Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 2004, 58: 195