Technical Data
N2254-01A
Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (FITC)
Description:
The Ly-6G locus encodes for a family of Ly-6 proteins including Ly-6G. This protein is expressed on mouse neutrophils, a subset of eosinophils, a differentiating pre-monocytes and plasmacytoid dendritic cells. N2254-01Ahas been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. Furthermore it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Functional studies. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Incubate 5x10e5 cells with 10ug/ml antibody (ref 4)
Immunohistochemistry (Paraffin): 1:50 Block with 20% normal rabbit serum (ref 5)
Immunohistochemistry (Frozen): 1:50 acetone fixation recommended (ref 7)
Functional Studies: Neutrophil depletion. MIce were treated with N2254-01A given intraperitoneally at a dose of 1mg, 6 hours before infection (ref 6)
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Mouse neutrophils

Recommended Negative Control:
Mouse thymocytes

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b6A608Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Purified BALB/c mouse neutrophils
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes mouse neutrophils (Ly-6G and Ly-6C).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lopez, A et al; Brit J Haematology 1984, 57: 489 2. McLaren, D et al; Parasite Immunol 1987, 9: 667 3. Folkard, S et al; Parasitology 1996, 112: 323 4. Van Lent, P et al; J Leukoc Biol 1997, 61: 267 5. Lubberts, E et al; J Immunol 1999, 163: 4546 6. Tacchini-Cottier, F et al; J Immunol 2000, 165: 628 7. Vries de, B et al; Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 2003, 170: 3883 8. Nagendra, S et al;. Cytometry A 2004, 58: 195