Technical Data
N2254-01F
Neutrophils Ly-6B.2 (Allotypic Marker) (Biotin)
Description:
Ly-6B.2 is a ~25-30kD GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kD (Rosas et al.2010).

This antibody has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Y3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10F229Affinity Purified
SizeStorageShippingSourceHost
100ug4C Do not freezeBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Cultured bone marrow cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes Ly-6B.2 Crossreactivity: 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; Swiss (PO) Strains. Negative/Weak reactivity for: A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hirsch, S. and Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by a monoclonal antibody 7/4. Immunogenetics 18: 229-239.2. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Opin. Microbiol. Immunol. 181: 1-37.