Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Y3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.
Storage and Stability:
Lyophilized powder may be stored at 4°C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
|100 Tests||4°C Do Not Freeze||Blue Ice||Mouse||Rat|
| Not determined|
|Cultured bone marrow cells.|
|Purified by Protein G affinity chromatography.|
|Supplied as a lyophilized powder from PBS, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|
|Recognizes a polymorphic 40kD antigen expressed by murine polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4:129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; Swiss (PO) Strains Negative/Weak for 7/4:A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Hirsch, S. and Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by a monoclonal antibody 7/4. Immunogenetics 18: 229-239.2. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Opin. Microbiol. Immunol. 181: 1-37.|