Technical Data
NIR2 (NIR-2, DRES9, Drosophila Retinal Degeneration B, Phosphatidylinositol Transfer Protein Membrane-associated, PITPNM, PYK2 N-terminal Domain-interacting Receptor 2, RD9, RDGB, RDGB1, RDGBA1)
NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis.
A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.

Cellular Localization: Cytoplasmic

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide: KARSISLKLDSEE, representing the C-terminus of the human protein (residues 1232-1244) according to NP_004901.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Mouse. Expected to crossreact with Human (100% homology).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lev S et al. Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein. Mol Cell Biol 19:2278-88 (1999).