Technical Data
N2700-03B
Nitrotyrosine (nTyr)
Description:
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase.
Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitrotyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.7ug/ml detects 5ug SIN-1 treated BSA using Goat anti-mouse IgG-HRP as the secondary antibody.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a7L25Affinity Purified
SizeStorageShippingSourceHost
25ug4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% azide, 50% glycerol.
Specificity:
Recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific. Species Crossreactivity: Human, mouse, rat and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Girault, I., et al., Free Radical Biology and Medicine 31(11): 1375-1387 (2001). 2. Gow, A.J., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 287(2): L262-L268 (2004). 3. Takemoto, K., et al., Acta Med. Okayama 61(1): 17-30 (2007). 4. Reynolds, M.R., et al., J. Nerosci. 26(42): 10,636-10,645 (2006). 5. Pfister, H., et al., Vet. Pathol. 39: 190-199 (2002). 6. Khan, J., et al., Biochem J. 330(2): 795-801 (1998).