Technical Data
Nod2 (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)
CARD15 (Nod2) is a member of the Nod1/Apaf-1 family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and a C-terminal regulatory domain. CARD15 induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It also confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Unlike Nod1, this homologue is primary expressed in monocytes. Defects in CARD15 are associated with Blau Syndrome (BS) and Crohn's Disease (CD), and are a cause of susceptibility to ulcerative colitis.

Positive Control: Human Peripheral Blood Mononucleocyte

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000.
Western Blot: 0.1-0.3ug/ml. ~100kD band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kD according to NP_071445.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide, KFRFTDRERHCSPTD, representing the internal region of the human protein according to NP_071445.1. Homology: Human, Mouse, Rat
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH7.2, 0.5% BSA.
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Keshav S.Paneth cells: leukocyte-like mediators of innate immunity in the intestine.J Leukoc Biol. 2006 Jun 22;PMID: 16793911