Technical Data
N3006-14F
NOD2, CT (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)
Description:
NOD2 is an intracellular protein of 100kD, which belongs to the NOD (nucleotide binding oligomerisation domain) family. The NOD family comprises at present more than 20 different mammalian NOD-LRR (leucine-rich repeat) proteins, which are involved in the regulation of apoptosis and the immune response. NOD2 has been shown to be constitutively or inducibly expressed in monocytes, macrophages, T and B cells, dendritic cells, as well as intestinal epithelial cells. A specific motif of peptidoglycan (PGN), muramyl dipeptide (MDP), has been identified as the sole ligand of NOD2. NOD2 and Toll-like receptors (TLR) are emerging as key mediators of innate host defence in the intestinal mucosa, crucially involved in maintaining mucosal and commensal homeostasis. Mutations in the gene encoding NOD2 are a major susceptibility factor underlying a significant subgroup of patients with Crohns disease.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
14aa at carboxy terminus of human NOD2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid, 0.02% sodium azide.
Specificity:
Recognizes human NOD2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inohara, N. and Nunez, G. (2003) NODs: intracellular proteins involved in inflammation and apoptosis. Nat. Rev. Immunol. 3:371-382 2. Cario, E. (2005) Bacterial interactions with cells of the intestinal mucosa: toll-like receptors and NOD2. Gut. 54:1182-1193. 3. Girardin, S. et al. (2003) Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J. Biol. Chem. 278:88698872.