Technical Data
N5350-01E
NOS I (NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS)
Description:
Alternate Name: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS

Domain Information: PDZ domain (Also known as DHR or GLGF); Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]; pfam02898, NO_synthase, Nitric oxide synthase, oxygenase domain

Gene Ontology Terms: nitric oxide synthase activity; calmodulin binding; electron transporter activity; nitric oxide biosynthesis; electron transport; synaptic transmission; muscle contraction; intracellular signaling cascade; cell-cell signaling; membrane fraction; oxidoreductase activity

Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested.
Western blot: Approx 160kD and 45kD bands seen in Mouse Brain extracts. Recommended for use at 0.5-2ug/ml.

Staining (0.5ug/ml) of Mouse Brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See N5350-01E-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
C-ESKKDTDEVFSS, C Terminus
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes Human, Rabbit NOS1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H., Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines., J Neurochem 1994 Jul;63(1):140-5