Technical Data
N6000-05M
NT-3 (Neurotrophin 3, Neurotrophin-3, NT3, NTF3, HDNF, MGC129711, Nerve Growth Factor 2, Nerve Growth Factor-2, NGF2, NGF-2, Neurotrophic Factor)
Description:
Seems to promotes the survival of visceral and proprioceptive sensory neurons.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1-10ug/ml
Western Blot: 1-10ug/ml,
Immunohistochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant human NT3. Cellular Localization: Secreted protein
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes human NT-3. Species Crossreactivity: rat and chicken. <1% crossreactivity to mouse NGF, rhBDNF; 5% to NT4/5
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhou et al. (1996) Mol. Neurobio. 13(3): 85-97. 2. Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55. 3. Tafreshi et al. (1998) Neurosci. 83(2): 373-80