		Technical Data
N7100-70	5'-Nucleotidase, Cytosolic II (NT5B) Recombinant, Human	50ug

Molecular Biology

Storage: -70°CShipping: Dry Ice

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.

Applications: Suitable for use in Suitable for use in ELISA and Western Blot (64.5kD). Other applications not tested.

IMPORTANT NOTE: This recombinant enzyme has NOT been tested with regard to biological activity.

Quality Control: SDS-PAGE

Protein GI #: 6912598

Protein Accession#: NP_036361

Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Important note: This recombinant enzyme has NOT been tested with regard to biological activity.

Source: Human, recombinant protein expressed in E. coli T7 tag at N-terminus (Full length (aa1-561).
Purity: Highly purified ( 95%).
Concentration: ~1mg/ml
Form: Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% sodium azide, 10mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.