Technical Data
Nutrient Agar w/MUG (Powder)
Microbiological Grade
Storage RT    Shipping RT
Formulation in grams per Liter:
Beef extract 3.0
Agar 15.0
MUG (4-Methylumbelliferyl- -D-glucuronide) 0.1
Total: 23.1g/Liter
Used with a fluorogenic assay for the detection of E. coli in water.

Beige, homogeneous, free-flowing powder

pH: 7.0 0.2

Microbiological Growth:
Enterococcus aerogenes ATCC 13048: Good Growth
Escherichia coli ATCC 11775:Good growth (Fluorescence +)
Escherichia coli ATCC 25922:Good growth (Fluorescence +)

Directions per Liter: Dissolve 23.1 grams per liter of distilled/deionized (DDI) water, heating with stirring boiling for one minute until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.