Technical Data
O3003-32
OAT 4 (Organic anion transporter 4)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Most recently, a novel member of the OAT family, OAT4, has been cloned and characterized. OAT4 (human 550 aa) is ~38-44% identical to other OATs. It is prominently expressed in the kidney and placenta. OAT4 interacted with chemically heterogeneous anionic compounds (nonsteroidal antiinflammatory drugs, diuretics, sulfobromopthalein, penicillin G, and bile acid salts). OAT4 may help in eliminating harmful anionic substances and protect the fetus.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml. O3003-30. Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL. Kidney or placenta can be used as positive control.
Immunohistochemistry: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide (18aa) from cytoplasmic, C-terminus of human OAT4 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human OAT4. No significant sequence homology is detected with other OATs or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhou F, 2004, Mol. Pharmacol., 65:1141-1147, WB 2. Ugele B, 2003, Am J Physiol. Endocrinology Metab: 284, 390-398. IHC 3. Cha SH et al (2000) J. Biol. Chem. 275, 4507-4512.