Technical Data
Oncostatin M, Recombinant, Human (OSM)
Molecular Biology Storage: -20CShipping: RT
Oncostatin M is an important growth regulating cytokine that affects, variably, a number of tumor and normal cells. Oncostatin M exerts inhibitory effects on the growth of A375 melanoma and other cancer cells but augments the growth of normal fibroblasts, AIDS related Kaposi sarcoma cells, and certain other cells. Oncostatin M is a 25.8kD protein containing 227 amino acid residues.

Biological Activity
USBio's human Oncostatin M is fully biologically active when compared to standards. The ED50 as determined by the dose dependent stimulation of the proliferation of human TF-1 cells is
2ng/ml, corresponding to a specific activity of 5x10e5 units/mg.

Responding Cells (partial list):
Tumor cells, certain other cells

Concentration Range:
For most in vitro applications: 0.1-10ng/ml

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume in sterile water and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, recombinant protein expressed in E. coli.
Purity: 98% by SDS-PAGE and HPLC analyses.
Endotoxin: 0.1ng per 1ug of Oncostatin M (1EU/ug).
Form: Form: Supplied as a lyophilized powder.
Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.